Metabolimetry - Flow Cytometry Core Facility

Assess the metabolic activity of your cells with our Seahorse XF analyser systems

Seahorse XF Analyser

The newest addition to the flow cytometry core facility, Agilent Seahorse XF technology measures cellular metabolism in live cells.

Living cells use one or both of the major energy producing pathways:

-          mitochondrial respiration

-          glycolysis

Seahorse XF technology measures cellular metabolism in live cells in real time.

Solid-state sensors measure extracellular flux (oxygen consumption rate (OCR) and extracellular acidification rate (ECAR)) providing insight into mitochondrial function and glycolytic activity.

Seahorse XF sensors simultaneously measure both OCR and ECAR in every well of either 96 (Seahorse XF96 system) or 24 well plates (Seahorse XF24 system). All platforms support injection of up to 4 unique compounds per well. At FCCF we have one XF96 at the medical school and one XF24 instrument at the Health Innovation Neighbourhood.